Functional Longevity

16:8 Intermittent Fasting: Benefits, Autophagy, Insulin Sensitivity & Fat Loss

16:8 Intermittent Fasting: Benefits, Autophagy, Insulin Sensitivity & Fat Loss

💡 What You Need to Know Right Away

16:8 fasting is a time-restricted eating pattern that helps you lose weight, improve blood sugar control, and support metabolic health by limiting food intake to 8 hours daily and fasting for 16 hours, including sleep.

Also known as: 16:8 intermittent fasting, 16/8 fasting, time-restricted eating (TRE), 8-hour eating window protocol

  • In people with obesity and type 2 diabetes, this eating pattern helped reduce weight by about 4% over 3 months[Evidence: B][1]
  • Research shows unwanted side effects occurred at similar rates in people fasting versus those not fasting, with serious adverse events rare at 0.3%[Evidence: A][7]
  • Studies indicate this eating pattern modestly improved blood sugar control in people with metabolic syndrome[Evidence: B][5]
  • In people with overweight or obesity, research shows weight decreased by about 3 pounds over several weeks[Evidence: A][2]

If you've been searching for a sustainable way to manage your weight without counting every calorie, you're not alone. Many people feel overwhelmed by complicated diet plans and restrictive eating rules. 16:8 fasting offers a simpler approach: eat during an 8-hour window, fast for 16 hours, and let your body do the rest.

It's common to wonder whether this eating pattern actually works and if it's safe for you. The good news is that current research from 2020 to 2025 provides clear evidence on both effectiveness and safety. In this guide, you'll learn exactly how 16:8 fasting works, what benefits to expect, potential risks to consider, and practical steps to get started. Whether you're looking for weight loss, better blood sugar control, or improved metabolic health, this evidence-based guide will help you make an informed decision.

❓ Quick Answers

Is 16:8 fasting safe?

For most healthy adults, 16:8 fasting is generally safe and well-tolerated. Research shows unwanted side effects like fatigue (14.5%), headache (13.5%), and dizziness (9.8%) occurred at similar rates in people fasting versus those not fasting[Evidence: A][7]. Serious adverse events were rare at 0.3%. However, people with existing heart disease, eating disorder history, or diabetes on medication should consult their doctor first.

How much weight can you lose with 16:8 fasting?

In people with overweight or obesity, research shows weight decreased by about 3 pounds over several weeks[Evidence: A][2]. Studies in people with obesity and type 2 diabetes found weight reduction of about 4% over 3 months[Evidence: B][1]. Results vary based on what you eat during your eating window and other lifestyle factors.

How long does 16:8 fasting take to work?

Studies suggest most people need 2-4 weeks for their body to adapt to the new eating schedule[Evidence: B][3]. Research shows measurable changes in weight and metabolic markers typically appear by 8-12 weeks. In people with type 2 diabetes, blood sugar improvements were seen alongside weight changes over 3 months[Evidence: A][12].

Is 16:8 fasting better than 14:10?

Studies suggest the 16:8 eating window was more effective for weight loss than the 14:10 eating window in people with obesity and type 2 diabetes[Evidence: B][1]. However, 14:10 may be easier for beginners to maintain. Research shows all intermittent fasting approaches help reduce weight compared to eating whenever desired[Evidence: A][6].

Can I do 16:8 fasting every day?

Yes, research shows most people were able to stick with the 16:8 eating schedule as a daily practice[Evidence: A][14]. Studies used both daily protocols and 3-5 days per week approaches with positive results. Daily practice offers consistency, but some studies used non-consecutive days with similar benefits.

What can I drink during the 16-hour fasting period?

During fasting hours, you can drink water, black coffee, and plain tea without breaking your fast. These beverages contain zero or near-zero calories. Adding milk, cream, or sugar to coffee or tea will break your fast. Sparkling water and herbal teas are also acceptable. The goal is to consume fewer than 5 calories during fasting hours.

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16:8 Method

Intermittent fasting isn't a diet, it's a pattern. Discover how 16 hours of rest can transform your metabolic health and mental clarity.

🔬 How Does 16:8 Fasting Work?

When you eat throughout the day, your body primarily burns glucose (sugar) from your most recent meal for energy. Think of your body like a hybrid car: it can run on two fuel sources. When you eat frequently, it runs on the "regular fuel" of glucose. But when you fast for 12-16 hours, your body switches to its "reserve tank," burning stored fat for energy instead.

This switch, called metabolic switching, typically happens 10-12 hours after your last meal. During the fasting period, your insulin levels drop, which signals your body to start using stored fat[Evidence: A][2]. Research shows this process improves insulin sensitivity and reduces insulin resistance over time[Evidence: A][8].

Studies indicate this eating pattern also reduced inflammation markers and improved cholesterol levels in people with metabolic syndrome[Evidence: A][15]. Research shows modest improvements in good cholesterol (HDL-C), though total cholesterol and bad cholesterol (LDL-C) changes were not significant[Evidence: A][9].

Timing matters too. Studies suggest eating earlier in the day (7 AM to 3 PM) was more effective for weight loss than eating later (noon to 8 PM) in people with obesity[Evidence: B][4]. This aligns with your body's natural circadian rhythm, where insulin sensitivity is higher in the morning. However, most people find evening windows more practical for social eating.

🧪 What to Expect: The Real User Experience

During the First Two Weeks

The first 1-2 weeks are the hardest. Expect intense hunger waves, typically 1-2 hours before your planned eating window opens. Many people describe "stomach growling," lightheadedness, and "thinking about food constantly." Hunger peaks around your usual breakfast time if you're using an evening eating window (noon to 8 PM). The good news: acute hunger typically lasts 20-30 minutes before subsiding if you stay distracted.

After 2-3 weeks, hunger becomes more manageable. Users report "knowing the hunger will pass," "distracted by morning productivity," and "clearer mental focus than expected." Mood also stabilizes. The initial irritability ("hangry" feeling) and difficulty concentrating fade as your body adapts.

Energy and Mood Patterns

During fasting hours, expect initial fatigue, grogginess, and "brain fog" until your first meal. After adaptation, many people report mental clarity, sustained energy, and no mid-morning crash compared to eating breakfast. During your eating window, you'll likely feel an energy surge after your first meal. Some experience post-meal drowsiness if the first meal is large or high in carbohydrates.

Practical Challenges

  • Social eating: Skipping breakfast meetings or morning coffee-with-pastries invitations creates awkwardness. Evening window users (noon to 8 PM) have easier social compatibility for dinners.
  • Black coffee adjustment: If you're used to cream and sugar, black coffee tastes bitter at first. By week 3-4, many report an "acquired taste." Some experience acidic stomach from coffee on empty stomach. Try cold brew (less acidic) or drink coffee closer to your eating window.
  • Nighttime snacking: The biggest adherence challenge. Brush your teeth right after your eating window closes to create a mental boundary.
  • Clock-watching: Expect to obsessively check the time in the last hour before your eating window opens. First meal feels more satisfying after waiting.

📊 How to Do 16:8 Fasting: Protocol and Timing

The 16:8 protocol involves fasting for 16 hours and eating within an 8-hour window daily. Most of the fasting happens while you sleep. Here are the research-backed protocols:

Purpose/Population Eating Window Duration Studied Evidence
Weight loss (obesity + T2D) 8-hour window daily 3 months [B][1]
Weight loss (overweight/obesity) Noon to 8 PM (8 hours) 12 weeks [B][3]
Metabolic syndrome 8-10 hour window 3 months [B][5]
Blood sugar control (T2D) 8-hour window Varies (8 RCTs) [A][12]
Early window (metabolic optimization) 7 AM to 3 PM 14 weeks [B][4]

Common Eating Window Options

Eating Window Best For Pros Cons
7 AM - 3 PM Morning people, metabolic optimization Aligns with circadian rhythm, better insulin sensitivity[4] Miss family dinners, difficult socially
10 AM - 6 PM Office workers, balanced schedule Late breakfast, can eat with colleagues at lunch Early dinner, may miss evening social eating
12 PM - 8 PM Most people, social compatibility Skip breakfast (easy), eat dinner with family Misses morning metabolic benefits
2 PM - 10 PM Night shift workers, late risers Late dinners possible, sleep shortly after eating May disrupt sleep, less metabolic benefit

How to Start

Begin with a 12-hour fast for the first week, then extend to 14 hours in week 2, and reach 16 hours by week 3. Studies show this gradual approach improves adherence[Evidence: A][14]. Choose an eating window that fits your lifestyle. Consistency matters more than perfection.

⚠️ Risks, Side Effects, and Warnings

For most healthy adults, 16:8 fasting is safe when done properly. Research shows unwanted side effects occurred at similar rates in people fasting versus those not fasting[Evidence: A][7]. Studies indicate no major unwanted side effects were reported in people following this eating pattern[Evidence: B][5].

Common Side Effects (First 2 Weeks)

  • Fatigue: 14.5% of people (similar to 16.2% in non-fasting control groups)[Evidence: A][7]
  • Headache: 13.5% of people (similar to 15.9% in controls)[Evidence: A][7]
  • Dizziness: 9.8% of people (similar to 9.4% in controls)[Evidence: A][7]
  • Constipation: 10.2% of people[Evidence: A][7]
  • Serious adverse events: Rare at 0.3%[Evidence: A][7]

Who Should NOT Try 16:8 Fasting

  • People with active eating disorders (anorexia, bulimia, binge eating disorder)
  • People with history of disordered eating
  • People with Type 1 diabetes
  • Pregnant or breastfeeding women
  • Children and adolescents under 18
  • People with BMI under 18.5 kg/m²
  • People with unstable cardiovascular disease
  • People taking diabetes medications (consult doctor for dose adjustment)

When to See a Doctor

Stop fasting and seek medical attention if you experience: severe dizziness or fainting, chest pain, rapid heartbeat, extreme fatigue that doesn't improve after eating, or signs of hypoglycemia (shakiness, confusion, sweating).

🥗 Practical Ways to Use 16:8 Fasting

How to Use This in Your Daily Life

Scenario 1: Weight Loss (Adults with Overweight/Obesity)

  • Protocol: 8-hour eating window daily
  • Duration: Minimum 8-12 weeks for measurable results[2]
  • Timing: Choose a consistent window (e.g., noon to 8 PM)
  • What to track: Weight weekly, waist circumference monthly
  • Expected results: About 3 pounds over several weeks[2]

Scenario 2: Blood Sugar Management (Type 2 Diabetes)

  • Protocol: 8-hour eating window (consult doctor for medication adjustment)
  • Duration: 3 months in clinical studies[1]
  • What to track: Blood glucose, HbA1c at baseline and 3 months
  • Expected results: Improved fasting blood sugar, modest HbA1c reduction[12]

What to Eat During Your 8-Hour Window

Focus on nutrient-dense foods: lean proteins (chicken, fish, eggs, legumes), vegetables, fruits, whole grains, and healthy fats (nuts, olive oil, avocado). Limit processed foods, sugary snacks, and high-calorie beverages. Research shows the quality of food matters as much as the timing[Evidence: A][14].

Common Mistakes to Avoid

  • Overeating during eating window: Fasting doesn't mean you can eat unlimited calories. Maintain reasonable portions.
  • Starting too aggressively: Jumping straight to 16-hour fasts causes more side effects. Ease in gradually over 2-3 weeks.
  • Ignoring hydration: Drink plenty of water during fasting hours. Dehydration causes headaches and fatigue.
  • Poor food choices: Filling your eating window with junk food undermines benefits. Quality matters.
  • Inconsistent timing: Studies used consistent daily schedules[1]. Varying your window day-to-day may reduce benefits.

Choosing the Right Approach for You

Not everyone responds the same way to 16:8 fasting. Here's how to determine if this approach is right for you and which eating window fits your lifestyle:

Is 16:8 Fasting Right For You?

  • Your health status: Best for generally healthy adults with overweight/obesity or metabolic concerns Why it matters: Studies focused on these populations[2]
  • Your eating history: No history of eating disorders or disordered eating Why it matters: Fasting can trigger restrictive behaviors[11]
  • Your schedule: Consistent daily routine where you can maintain regular eating times Why it matters: Consistency improves adherence and results
  • Your medications: Not on diabetes medications requiring dose adjustment (or willing to work with doctor) Why it matters: Fasting affects blood sugar and medication needs

How to Choose Your Eating Window

  • Morning person? Consider 7 AM - 3 PM or 8 AM - 4 PM Why it matters: Research shows earlier windows have metabolic advantages[4]
  • Family dinners important? Consider noon - 8 PM or 1 PM - 9 PM Why it matters: Social eating improves long-term adherence
  • Work schedule constraints? Match your window to when meals are most convenient Why it matters: Practical sustainability beats optimal timing

Red Flags: When to Reconsider

  • Obsessive thoughts about food: If fasting causes constant preoccupation with eating, stop and reassess
  • Binge eating after fasting: If you overeat uncontrollably when your window opens, this approach may not suit you
  • Significant mood changes: Persistent irritability, anxiety, or depression beyond the first 2 weeks
  • Physical symptoms: Fainting, severe weakness, or symptoms that don't improve with eating

How 16:8 Compares to Other Fasting Methods: What to Know

16:8 is one of several intermittent fasting approaches. Research shows all intermittent fasting strategies help reduce weight compared to eating whenever desired[Evidence: A][6]. Here's how they compare:

Feature 16:8 TRE 14:10 TRE 5:2 Diet Alternate Day Fasting
Fasting Hours (Daily) 16 hours 14 hours Not daily (2 days/week) 24+ hours every other day
Eating Window 8 hours 10 hours Normal eating 5 days Alternating normal/restricted
Weight Loss Effectiveness About 3-4% in studies[1] About 3.15% in studies[1] Comparable to 16:8[6] Slightly superior short-term[6]
Ease of Adherence Moderate Easier (longer window) Variable (severe restriction 2 days) More difficult (full fast days)
Best For Daily routine, weight loss Beginners, flexibility needed Those who prefer occasional restriction Aggressive short-term results
Social Compatibility Good (daily eating) Better (longer window) Poor on fast days Poor on fast days

Early vs. Late Eating Windows

Research shows eating earlier in the day (7 AM to 3 PM) was more effective for weight loss than eating later (noon to 8 PM) and improved blood pressure and mood scores[Evidence: B][4]. However, most people find later windows easier to maintain socially. Choose based on your lifestyle priorities.

What The Evidence Shows (And Doesn't Show)

What Research Suggests

  • In people with overweight or obesity, 16:8 fasting helped reduce weight by about 3 pounds (1.48 kg) over several weeks, based on meta-analysis of multiple trials[Evidence: A][2]
  • Research shows the 16:8 eating pattern produced similar weight loss results as daily calorie restriction (99 RCTs, 6,582 adults)[Evidence: A][6]
  • Studies indicate this approach improved insulin resistance with high certainty (GRADE assessment)[Evidence: A][15]
  • Serious adverse events were rare at 0.3%, and common side effects (fatigue, headache, dizziness) occurred at similar rates in fasting vs. non-fasting groups[Evidence: A][7]
  • In people with type 2 diabetes, blood sugar control improved with HbA1c reduction and increased time in healthy range[Evidence: A][12]

What's NOT Yet Proven

  • Long-term outcomes beyond 1 year are not established. Most studies lasted 2-16 weeks; the longest follow-up was 52 weeks in network meta-analysis[6]
  • Optimal eating window timing not conclusively established. Early windows (7 AM-3 PM) showed some advantages, but most studies used noon-8 PM[4]
  • Weight loss magnitude is modest (2-4% body weight). This matches continuous calorie restriction but doesn't exceed it[6]
  • Muscle mass preservation unclear. One observational study suggested possible lean mass reduction with strict 8-hour windows[10]
  • Effects in specific populations (children, pregnant women, elite athletes) not studied

Where Caution Is Needed

  • Cardiovascular mortality signal: A 2024 observational study found 91% higher heart-related death risk with 8-hour eating windows. Causality not established. This requires further research[Evidence: D][10]
  • Eating disorder risk in vulnerable populations: Clinical guidelines warn against fasting in people with eating disorder history, adolescents, and young adults[Evidence: D][11]
  • Cholesterol effects mixed: HDL-C (good cholesterol) showed modest improvement, but total cholesterol and LDL-C did not significantly change[Evidence: A][9]
  • Benefits may diminish in longer trials. Network meta-analysis noted moderate duration benefits exceeded long-term trials[6]

Should YOU Try This?

Best suited for: Generally healthy adults with overweight or obesity seeking a sustainable eating pattern that doesn't require calorie counting. People with metabolic syndrome or type 2 diabetes (with medical supervision) may benefit from improved blood sugar control.

Not recommended for: People with eating disorder history, adolescents, pregnant or breastfeeding women, people with Type 1 diabetes, people with existing cardiovascular disease (discuss with doctor first), anyone with BMI under 18.5.

Realistic timeline: 2-3 weeks for adaptation, 8-12 weeks for measurable weight and metabolic changes. Expect modest (not dramatic) improvements.

When to consult a professional: Before starting if you take medications (especially for diabetes or blood pressure), have any chronic health conditions, experience persistent side effects beyond 2 weeks, or notice signs of disordered eating patterns developing.

Frequently Asked Questions

Can I drink coffee with milk during the fasting window?

Technically, adding milk or cream to coffee breaks your fast because it adds calories. A splash of milk (about 1 tablespoon) adds 5-10 calories, which may be acceptable for some goals but will trigger insulin response. For strict metabolic benefits, stick to black coffee. Many people find they acquire a taste for black coffee after 2-3 weeks. If you struggle with black coffee, try cold brew (less bitter) or wait to have coffee with milk until your eating window opens.

Can I exercise during the fasting period?

Yes, but timing matters. Fasted workouts feel harder during the first 2 weeks of adaptation. After your body adjusts, many people report 'running on stored energy efficiently.' Light to moderate exercise (walking, yoga) is generally fine fasted. For intense workouts (weight training, HIIT), you may perform better eating first. Experiment with exercising at the end of your fast (just before eating) versus during your fed window to find what works best for you.

Will 16:8 fasting slow my metabolism?

Research does not support the idea that intermittent fasting slows metabolism when done as studied (8-16 hours of daily fasting). Studies show metabolic rate remained stable and weight loss came primarily from fat mass reduction . This differs from prolonged caloric restriction, which can lower metabolic rate over time. However, research on effects beyond 16 weeks is limited.

Is 16:8 fasting safe for women?

Studies included women and showed similar benefits for weight and metabolic outcomes . However, clinical guidelines note that women may be at higher risk for developing eating problems with intermittent fasting . Some women report menstrual irregularities with strict fasting. If you notice hormonal changes, consider a milder approach (14:10) or consult your healthcare provider.

What if I feel too hungry to continue?

Hunger is normal during adaptation and typically subsides after 2-3 weeks. If hunger feels unmanageable: drink water (thirst mimics hunger), have black coffee or tea, stay busy with activities, or shorten your fast slightly (try 14:10 first). If hunger persists beyond 3-4 weeks or causes significant distress, this approach may not be right for you. Never force through severe symptoms. Research shows the protocol was well-tolerated, but individual responses vary .

Can I take supplements or medications during fasting?

Most medications should be taken as prescribed, regardless of fasting schedule. Consult your doctor about timing, especially for medications that require food (to prevent stomach upset) or affect blood sugar. Fat-soluble vitamins (A, D, E, K) absorb better with food, so take them during your eating window. Water-soluble supplements can be taken anytime. If you take diabetes medications, work with your doctor on dose adjustment, as fasting changes blood sugar patterns.

How do I handle social situations with fasting?

Flexibility helps long-term success. Options include: adjusting your eating window for special occasions, scheduling social meals during your regular window, or simply participating socially without eating (coffee meetings during fasting hours). Research shows high compliance rates even with occasional schedule variations . One off-schedule meal won't undo your progress. Return to your regular schedule the next day.

What results should I expect at 1 month?

At one month, expect: weight reduction of 2-4 pounds if overweight/obese , reduced hunger and cravings (adaptation complete), improved energy levels after the initial adjustment period, and beginning of metabolic improvements. Blood sugar and cholesterol changes may not be measurable until 8-12 weeks. Don't expect dramatic transformation. Slow, sustainable progress is typical and healthy.

Should I count calories while doing 16:8 fasting?

Studies suggest the 16:8 eating pattern produced similar weight loss results as daily calorie restriction . This means time restriction naturally reduces intake for most people without counting. However, if you're not seeing results after 4-6 weeks, you may be consuming too many calories during your eating window. At that point, briefly tracking intake can reveal whether portion sizes are appropriate. Focus first on food quality, then quantity if needed.

Our Accuracy Commitment and Editorial Principles

At Biochron, we take health information seriously. Every claim in this article is supported by peer-reviewed scientific evidence from reputable sources published in 2015 or later. We use a rigorous evidence-grading system to help you understand the strength of research behind each statement:


  • [Evidence: A] = Systematic review or meta-analysis (strongest evidence)
  • [Evidence: B] = Randomized controlled trial (RCT)
  • [Evidence: C] = Cohort or case-control study
  • [Evidence: D] = Expert opinion or clinical guideline

Our editorial team follows strict guidelines: we never exaggerate health claims, we clearly distinguish between correlation and causation, we update content regularly as new research emerges, and we transparently note when evidence is limited or conflicting. For our complete editorial standards, visit our Editorial Principles page.


This article is for informational purposes only and does not constitute medical advice. Always consult qualified healthcare professionals before making changes to your health regimen, especially if you have medical conditions or take medications.

References

  1. 1 . Effect of intermittent fasting 16:8 and 14:10 compared with control-group on weight reduction and metabolic outcomes in obesity with type 2 diabetes patients: A randomized controlled trial, Journal of Diabetes Investigation, 2024, PubMed [Evidence: B]
  2. 2 . Is time-restricted eating (8/16) beneficial for body weight and metabolism of obese and overweight adults? A systematic review and meta-analysis of randomized controlled trials, Food Science & Nutrition, 2023, PubMed [Evidence: A]
  3. 3 . Effects of Time-Restricted Eating on Weight Loss and Other Metabolic Parameters in Women and Men With Overweight and Obesity: The TREAT Randomized Clinical Trial, JAMA Internal Medicine, 2020, PubMed [Evidence: B]
  4. 4 . Effectiveness of Early Time-Restricted Eating for Weight Loss, Fat Loss, and Cardiometabolic Health in Adults With Obesity: A Randomized Clinical Trial, JAMA Internal Medicine, 2022, PubMed [Evidence: B]
  5. 5 . Time-Restricted Eating in Adults With Metabolic Syndrome: A Randomized Controlled Trial, Annals of Internal Medicine, 2024, PubMed [Evidence: B]
  6. 6 . Intermittent fasting strategies and their effects on body weight and other cardiometabolic risk factors: systematic review and network meta-analysis of randomised clinical trials, BMJ, 2025, PubMed [Evidence: A]
  7. 7 . Adverse events profile associated with intermittent fasting in adults with overweight or obesity: a systematic review and meta-analysis of randomized controlled trials, Nutrition Journal, 2024, PubMed [Evidence: A]
  8. 8 . The effect of intermittent fasting on insulin resistance, lipid profile, and inflammation on metabolic syndrome: a GRADE assessed systematic review and meta-analysis, Journal of Health, Population and Nutrition, 2025, PubMed [Evidence: A]
  9. 9 . Effect of 8-Hour Time-Restricted Eating (16/8 TRE) on Glucose Metabolism and Lipid Profile in Adults: A Systematic Review and Meta-Analysis, Nutrition Reviews, 2025, DOI [Evidence: A]
  10. 10 . 8-hour time-restricted eating linked to a 91% higher risk of cardiovascular death, American Heart Association Scientific Sessions 2024 (Abstract P192), 2024, AHA Newsroom [Evidence: D]
  11. 11 . Intermittent fasting: consider the risks of disordered eating for your patient, Clinical Diabetes & Endocrinology, 2023, PubMed [Evidence: D]
  12. 12 . Time-Restricted Eating Improves Glycemic Control in Patients with Type 2 Diabetes: A Meta-Analysis and Systematic Review, International Journal of Molecular Sciences, 2025, PubMed [Evidence: A]
  13. 13 . What are the effects of time-restricted eating upon metabolic health outcomes in individuals with metabolic syndrome: A scoping review, Physiological Reports, 2025, DOI [Evidence: D]
  14. 14 . Is time-restricted eating a healthy choice to lose weight? Investigating by qualitative analysis of Instagram posts and systematic reviews with meta-analysis, Digital Health, 2025, DOI [Evidence: A]
  15. 15 . Intermittent fasting improves metabolic outcomes in metabolic syndrome: a systematic review and meta-analysis with GRADE evaluation, Frontiers in Nutrition, 2025, DOI [Evidence: A]

Medical Disclaimer


This content is for informational and educational purposes only. It is not intended to provide medical advice or to take the place of such advice or treatment from a personal physician. All readers are advised to consult their doctors or qualified health professionals regarding specific health questions and before making any changes to their health routine, including starting new supplements.

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